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XLGraph
What is the purpose of XLGraph?
The XLGraph program is:
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A general purpose plotting tool for AUC scan data
(optionally after subtraction of
baseline scans). XLGraph also has an "automatic graphing" utility that
is useful for displaying new XL/A or XL/I scans as they are acquired, and
also for automatically printing out graphs of all the data at the end of an
experiment for documentation purposes. This function does not work with
'live' data from the new Optima AUC, but can plot or print Optima scans
after they are exported.
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A utility to transform the raw data plots to help visualize and
interpret what is happening in the AUC experiment. These
transformations include:
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plotting equilibrium scans as ln(signal) vs. radius2/2 to
create a plot where the slope is proportional to the buoyant molecular
weight
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converting the radius axis of velocity scans into the apparent
sedimentation coefficient (s*) to get a rough idea of what
sedimentation coefficients are present in the sample
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for scans where the meniscus region has been cleared but a plateau region
is still present, transform the scans into the integral sedimentation
coefficient distribution, G(s*)
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subtract velocity scans in pairs and plot the resultant dc/dt
versus sedimentation coefficient curves
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plot interference scans after removing integral fringe jumps
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display the scans from a velocity run as a 'movie' graph to follow the
boundary movement
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A utility for performing certain mathematical operations on raw AUC
scans and then writing out the resultant data as new scan files.
These operations, include:
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Averaging together multiple scans to improve the signal/noise ratio (for
example several scans recorded after a sedimentation equilibrium sample
has already reached equilibrium
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Transforming the scan data by multiplying the signal by a constant (for
example by -1 to correct for a cell being loaded upside-down), and/or by
shifting the radial values by a constant (for example to correct for a
known calibration error).
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Altering the comment stored in the scan headers
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System Requirements
XLGraph runs under Windows XP, Vista, 7, 8, or 10. Use via
Windows dual-boot configurations on MacIntosh systems usually works fine, but is
not officially supported.
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